Aurintricarboxylic acid inhibition of the binding of phenylalanyl-tRNAa to rat liver ribosomal subunits.

Evidence for one functional phenylalanyl-tRNA binding site on the 30S ribosomal subunit.

NH(2) terminal analysis of polyphenylalanine formed from 30S subunit-bound (14)C-phenylalanyl-tRNA suggests that there is only one site in a 30S subunit for specific binding of phenylalanyl-tRNA. Assuming that no movement of the bound phenylalanyl-tRNA takes place during association of 30S subunits with 50S ribosomal subunits, the binding site of the 30S ribosomal subunit corresponds to site 2 ...

Aurintricarboxylic acid is a potent inhibitor of phosphofructokinase.

Aurintricarboxylic acid (ATA) was found to be a very potent inhibitor of purified rabbit liver phosphofructokinase (PFK), giving 50% inhibition at 0.2 microM. The inhibition was in a manner consistent with interaction at the citrate-inhibitory site of the enzyme. The data suggest that inhibition of PFK by ATA was not due to denaturation of the enzyme or the irreversible binding of inhibitor, si...

Factor-promoted dissociation of free ribosomes in a rabbit reticulocyte lysate system: inhibition and requirement for an energy source.

A factor that promoted the dissociation of ribosomes into ribosomal subunits in a complete globin-synthesizing system was isolated from rabbit reticulocyte ribosomes. The factor stimulated both globin synthesis and the association of the small ribosomal subunit with polyribosomes. The dissociation of free ribosomes by the factor could be followed as an independent reaction in the presence of a ...

Effect of 7,12-dimethylbenz(a)anthracene on the binding of aminoacyl transfer RNA in rat liver.

Homogenates of the liver of female rats obtained 2 or 7 days or 3 months after the i.v. injection of 7,12-dimethylbenz(a)anthracene were used to prepare ribosomes and postmicrosomal supernatant fractions and to prepare 0.5 M KCl salt wash fractions of the 40 S ribosomal subunits. The activity of the ribosomes was unchanged by 7,12-dimethylbenz(a)anthracene. The activity of the supernatant prepa...

Release of the nonribosomal proteins from the mammalian native 40 S ribosomal subunit by aurintricarboxylic acid.